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周恩民 
发布时间:2016-04-15  浏览次数:
姓名

周恩民

性别
学科专业

预防兽医系

联系信箱

zhouem@nwsuaf.edu.cn

出生日期

1957.6

籍贯

内蒙古

教授级别

二级

毕业院校

加拿大明尼多巴大学医学院

研究方向: 猪繁殖与呼吸综合征防治 首次发现了蓝耳病病毒自身抗独特型抗体的存在。该抗体模拟蓝耳病病毒的表面抗原,识别抗该表面抗原的抗体。相继制备了单克隆抗独特型抗体,并由此鉴定出了一个新型的蓝耳病病毒受体,发现了该受体的阻断抑制剂。获得了国家发明专利。 戊型肝炎病毒研究 首次证明禽HEV(美国分离株)的 ORF2 蛋白免疫后能够产生中和抗体并可以防止HEV的感染。ORF2 蛋白的抗原表位可以作为有效的免疫诊断抗原,用来鉴别人和禽HEV抗原决定簇。研究表明禽与人、猪HEV具有某些相同的抗原决定簇。最近首次在中国患有肝脾肿大综合征的鸡病料中分离到禽HEV,已完成全基因组鉴定。 先后在 J. Immunol. (免疫学杂志)、J. Virol.(病毒学杂志)、J. Gen. Virol.(普通病毒学杂志)J. Virol. Methods(病毒方法学杂志)等刊物上发表67篇论文,在国际会议上发表论文70余篇。
个人简历: 2010年7月至现在: 西北农林科技大学“后稷学者”特聘教授、动物医学院院长 2011年入选陕西省“百人计划”; 2007年7月至2010年6月: 山东省“泰山学者海外特聘专家”; 山东农业大学动物科技学院、动物医学院副院长; 2006年5月至2007年7月: “龙江学者” 特聘教授:黑龙江八一农垦大学动物科技学院 2000年6月至2006年4月: 副教授、主任:美国衣阿华州立大学兽医学院兽医诊断和动物生产医学系 2002年12月至2005年12月: 客座教授: 东北农业大学动物医学院 1998年9月至2000年6月: 副教授:加拿大明尼多巴大学农学院动物科学系,兼职 1992年4月至2000年5月: 主任、高级研究员:加拿大国家食品检验检疫总局国家外来病中心免疫诊断实验室 1995年1月1998年12月: 客座副教授:加拿大渥太华大学微生物免疫学系 1985年9月1988年8月: 访问学者,博士后:美国德可萨思州圣安东尼奥西南生物医学研究所病毒免疫学系 1978年9月至1985年8月: 助教:包头医学院微生物教研室
代表性论著: 论文: (*为通讯作者):(共 68篇) 1.Qin Zhao, En-Min Zhou*, Shi-Wei Dong, Hong-Kai Qiu, Lu Zhang, Shou-Bin Hu, Fei-Fei Zhao, Shi-Jin Jiang, and Ya-Ni Sun. Genetic identification and sequence analysis of avian hepatitis E virus from chickens in China. Accepted for publication at Emerging and Infectious Diseases. 2010:. 2.赵钦,周恩民*,孙培明,董施伟,张璐,孙亚妮,姜世金。我国鸡群禽戊型肝炎病毒RT-PCR检测与鉴定。中国预防兽医学报(Chinese J Pre Vet Med),2010: 3.Shao-Ning Liu, Xing-Xiao Zhang, Jin-Feng Zou, Zhi-Jing Xie, Yan-Li Zhu, Qin Zhao, En-min Zhou, Shi-Jin Jiang. Development of an indirect ELISA for the detection of duck circovirus infection in duck flocks. Vet Microbiol. 2010, online March 17 4.Zhao Q, Sun YN, Zhang XX, Kong YB, Xie ZJ, Zhu YL, Zhou EM, Jiang SJ. Evaluation of two experimental infection models for Avibacterium paragallinarum. Vet Microbiol. 2010; 141(1-2):68-72. 5.李晓静,周恩民*,赵钦,肖一红,马利宏。一株猪繁殖与呼吸综合征病毒的分离及ORF5基因序列与国内外毒株的分析和比较。西南农业学报(Southwest China Journal of Agricultural Sciences).2009; 22(4):1103-1109. 6.张红霞,荆胜涛,周恩民*,孙培明,董施伟,李艳菊。人血清中抗禽戊型肝炎病毒抗体的检测与鉴定。中国卫生检验杂志 (Chin J Health Lab Technol). 2009; 19(8):1741-1744. 7.周恩民,荆胜涛,彭军,孙培明,张璐,董施伟。禽戊性肝炎病毒人兽共感染的可能性研究。中国家禽. 2009; 31(1):5-9. 8.冉旭华,闻晓波,孟凡,周恩民。猪细小病毒NS1蛋白主要抗原表位区的原核表达及间接ELISA方法的建立。中国兽医学报(Chinese J Vet Sci)。2009; 29(8): 9.周恩民*,荆胜涛,张璐,董施伟,彭军,孙培明。禽戊型肝炎研究进展。山东畜牧兽医。2008; 11:1-3. 10.张璐,周恩民*,崔治中,董施伟,荆胜涛,彭军,孙培明,孙淑红,秦卓明。禽戊型肝炎血清学调查以及与肝脾肿大综合征关系的研究。中国家禽。2008; 30(20): 22-24. 11.冉旭华,孟凡,闻晓波,单玉波,周恩民。猪细小病毒NS1蛋白主要抗原表位区的原核表达。生物技术(Biaotechnology)。2008; 18(2):18-20. 12.Zhou E-M*, Xiao YH, Shi YF, Li XJ, Ma LH, Jing ST. Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus. J Virol Methods. 2008; 149:300-308. Impact factor: 2.097. 13.Zhou E-M*, Guo H, Huang FF, Sun ZF, Meng XJ. Identification of two neutralization epitopes on the capsid protein of avian hepatitis E virus (avian HEV). J Gen Virol. 2008; 89:500-508. Impact factor: 3.300. (Cited 1) 14.Guo H, Zhou EM, Sun ZF, Meng XJ. Immunodominant epitopes mapped by synthetic peptides on the capsid protein of avian hepatitis E virus are non-protective. Viral Immunol. 2008; 21(1):61-7. (Cited 1) 15.Boyer TC, Ward MP, Wallace RL, Zhou E-M, Singer RS. Exploratory spatial data analysis of regional seroprevalence of antibodies against epizootic hemorrhagic disease virus in cattle from Illinois and Indiana. Ame J Vet Res. 2008; 69(10):1286-1293. 16.Guo H, Zhou E-M*, Sun ZF, Meng XJ. Protection of chickens against avian hepatitis E virus (avian HEV) infection by immunization with recombinant avian HEV capsid protein. Vaccine. 2007; 25:2892-2899. Impact factor: 2.822. 17.Guo H, Zhou E-M*, Sun ZF, Meng XJ. Egg whites from eggs of chickens experimentally infected with avian hepatitis E virus contain infectious virus but evidence of complete vertical transmission is lacking. J Gen Virol. 2006; 88:1532-1537. Impact factor: 3.300. 18.Guo H, Zhou E-M,* Sun ZF, Meng XJ, Halbur PG. Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV. J Gen Virol. 2006; 87(1):217-223. Impact factor: 3.300. (Cited 1) 19.Trampel DW, Zhou E-M, Yoon KJ, Koehler KJ. Detection of antibodies in serum and egg yolk following infection of chickens with an H6N2 avian influenza virus. J Vet Diagn Invest. 2006; 18(5):437-442. Impact factor: 1.609. (Cited 1) 20.Opriessnig T, McKeown NE, Zhou E-M, Meng XJ, Halbur PG. Genetic and experimental comparison of porcine circovirus type 2 (PCV2) isolates from cases with and without PCV2-associated lesions provides evidence for differences in virulence. J Gen Virol. 2006; 87(Pt 10):2923-2932. Impact factor: 3.300. (Cited 3) 21.Ameri-Mahabadi M and Zhou E-M. Idiotype and anti-idiotype: a review. Comp Clin Pathol. 2006; 14:171-178. 22.Ameri-Mahabadi M, Zhou E-M*, Hsu W. A Western blot immunoassay as a confirmatory test for the presence of anti-mycoplasma hyopneumoniae antibodies in swine serum. J Vet Diagn Invest. 2006; 18(2):198-201. Impact factor: 1.609. 23.Ameri-Mahabadi M, Zhou E-M,* Hsu W. Comparison of two swine mycoplasma hyopneumoniae enzyme-linked Immunosorbent assays for detection of antibodies from vaccinated pigs and field serum samples. J Vet Diagn Invest. 2005; 17: 61-64. Impact factor: 1.609. (Cited 2) 24.Clavijo A, Zhou E-M, Hole K, Galic B, Kitching P. Development and use of a biotinylated 3ABC recombinant protein in a solid-phase competitive ELISA for the detection of antibodies against foot-and-mouth disease virus. J Virol Methods. 2004; 15:217-227. Impact factor: 1.938. (Cited 1) 25.Zhou E-M*, Clavijo A, Jiang Z, Ameri-Mahabadi M, Zimmerman JJ. Induction of auto-anti-idiotypic antibodies specific for antibodies to matrix and envelope glycoprotein from pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2004; 101:49-59. Impact factor: 1.659. 26.Skibbe D, Zhou E-M*, Janke BH. Comparison of a commercial ELISA with hemagglutination inhibition assay for serdiagnosis of swine influenza virus (H1N1) infection. J Vet Diagn Invest. 2004; 16:86-89. Impact factor: 1.609. 27.Zhou J-Y, Chen Q-X, Ye J-X, Zhu J-X, Zheng X-J, Shang S-B, Chen T-F, Mu A-X, Zhou E-M. Serological investigation of porcine circovirus 2 infection. Chinese J Vet Med. 24:1-3. (猪二型圆环病毒的血清学调查。<<中国兽医学杂志>>,2004年第24期,1-3页)。 28.Zhou J-Y, Wu J-X, Cheng L-Q, Zheng X-J, Gong H, Shang S-B and Zhou E-M*. Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes. J Virol. 2003; 77(16):9090-9093. Impact factor: 5.241. (Cited 2) 29.Jiang ZJ, Zhou E-M*, Ameri-Mahabadi M, Zimmerman JJ and Platt KB. Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein. Vet Immunol Immunopathol. 2003; 92:125-135. Impact factor: 1.659. 30.Clavijo A, Tresnan DB, Jolie R, Zhou E-M. Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus. Can J Vet Res. 2002; 66:117-121. Impact factor: 1.114. (Cited 5) 31.Zhou E-M*, Ridd D, Riva J, Fernando L, Clavijo A. Development and evaluation of an IgM-capture ELISA for detection of recent infection with bluetongue virus in cattle. J Virol Methods. 2001; 91:175-182. Impact factor: 1.938. 32.Zhou E-M*, Riva J, Clavijo A. Development of an immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay for detection of equine and swine IgM antibodies to vesicular stomatitis virus. Clin Diagn Lab Immunol. 2001; 8:475-481. Impact factor: 1.654. (Cited 1) 33.Clavijo A, Lin M, Riva J, Zhou E-M. Application of competitive enzyme-linked immunosorbent assay for the serological diagnosis of classical swine fever virus infection. J Vet Diagn Invest. 2001; 13:357-360. Impact factor: 1.609. (Cited 1) 34.Clavijo A, Lin M, Riva J, Mallory M, Lin F, Zhou E-M. Development of a competitive ELISA using a truncated E2 recombinant protein as antigen for detection of antibodies to classical swine fever virus. Res Vet Sci. 2001; 70:1-7. Impact factor: 1.011. 35.Clavijo A, Riva J, Copps J, Robinson Y, Zhou E-M. Assessment of the pathogenicity of an emu-origin influenza A H5 virus in ostriches (Struthio camelus). Avian Pathol. 2001; 30:83-89. Impact factor: 1.514. 36.Pasick J, Handel, K, Zhou E-M, Clavijo A, Coates J, Robinson Y, and Lincoln B. Incursion of epizootic hemorrhagic disease into the Okanogan Valley, British Columbia in 1999. Can Vet J. 2001; 42:207-209. Impact factor: 0.742. 37.Clavijo A, Munroe F, Zhou E-M, Booth TF, Roblesky K. Incursion of bluetongue into the Okanogan Valley, British Columbia. Can Vet J. 2000; 41:312-314. Impact factor: 0.742. 38.Heckert A, McIsaac M., Chan M, Zhou E-M. Experimental infection of emus (Dromaius novaehollandiae) with avian influenza virus of various virulence: clinical signs, virus shedding and serology. Avian Pathol. 1999; 23:13-16. Impact factor: 1.514. 39.Zhou E-M*, Afshar A. Characterization of monoclonal antibodies to epizootic hemorrhagic disease of deer virus (EHDV) and bluetongue virus by immunization of mice with EHDV recombinant VP7 antigen. Res Vet Sci. 1999; 66:247-252. Impact factor: 1.011. 40. Zhou E-M. Anti-idiotype technique: an alternative approach for immunodiagnosis of bluetongue. Vet Immunol Immunopathol. 1999; 72:237-242. Impact factor: 1.659. 41. Wright P, Zhou E-M. Developments in international standardization. Vet Immunol Immunopathol. 1999; 72:243-248. Impact factor: 1.659. 42. Zhou E-M*, Chan M, Heckert RA, Riva J, Cantin M-F. Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis. 1998; 42:517-522. Impact factor: 1.151. 43. Clavijo A, Zhou E-M, Vydelingum S, Heckert R. Development and evaluation of a novel antigen capture assay for the detection of classical swine fever virus antigens. Vet Microbiol. 1998; 60:155-168. Impact factor: 1.727. (Cited 5) 44. Zhou E-M*, Chan M, McIsaac M, Heckert R. Evaluation of antibody responses of emus (Dromaius novaehollandiae) to avian influenza virus infection. Avian Dis. 1998; 42:757-761. Impact factor: 1.151. 45. Xu G, Wilson W, Mecham J, Murphy K, Zhou E-M, Tabachnick W. VP7: an attachment protein of bluetongue virus for cellular receptors in Culicoides variipennes. J Gen Virol. 1997; 78:1617-1623. Impact factor: 3.300. (Cited 5) 46. Prud'homme I., Zhou E-M, Traykova M, Trotter H, Chan M, Afshar A, Harding MJ. Production of a baculovirus-derived gp50 protein and utilization in a competitive enzyme-linked immunosorbent assay for the serodiagnosis of pseudorabies virus. Can J Vet Res. 1997; 61:286-291. Impact factor: 1.114. 47. Zhou E-M*, Chan MKH. Immunoblot assay as a confirmatory test of competitive ELISA for serodiagnosis of bluetongue. J Vet Diagn Invest. 1996; 8(3):365-367. Impact factor: 1.609. 48. Lin M, Zhou E-M*. Induction of antibodies to the bluetongue virus core polypeptide VP7 in sheep by internal image rabbit anti-idiotypic antibodies. Viral Immunol. 1996; 9(1):35-43. Impact factor: 1.625. (Cited 1) 49. Lin M, Zhou E-M*. Evidence that two distinct populations of rabbit anti-idiotypic antibodies induced by three monoclonal antibodies specific for bluetongue virus core protein VP7. Microbiol Immunol. 1996; 40(4):295-302. 50. Lin M, Zhou E-M*. Biological mimicry of the bluetongue virus core protein VP7 by rabbit anti-idiotype. Microbiol Immunol. 1996; 40(6):435-441. 51. Zhou E-M, Kisil FT. Regulation of antibody formation to Ryegrass pollen allergen Lol pIV by an internal image anti-idiotypic monoclonal antibody. Immunol. 1995; 84:343-349. Impact factor: 2.729. 52. Zhou E-M*, Huang W. Anti-idiotypic antibody as a potential serodiagnostic reagent detecting bluetongue virus infection. J Clin Microbiol. 1995; 33(4):850-854. Impact factor: 3.565. 53. Zhou E-M*, Afshar A. Comparison of Freund's adjuvant and TiterMaxTM in inducing anti-idiotype to idiotypic antibodies against pseudorabies virus antigens. Vet Immunol Immunopathol. 1995; 48:113-122. Impact factor: 1.659. 54. Lin M, Zhou E-M*.Internal image anti-idiotypic antibody detects sheep antibodies to the bluetongue virus core protein VP7. Immunotechnol. 1995; 1:151-155. Impact factor: 2.598. 55. Zhou E-M*, Kisil FT. Antibody responses to allergen Lol pIV are suppressed following adoptive transfer of B lymphocytes from the internal image anti-idiotypic antibody treated mice. Cell Immunol. 1995; 165:77-83. Impact factor: 1.988. 56.Zhou E-M*, Afshar A, Heckert RA, Nielsen K. Anti-idiotypic antibodies generated by sequential immunization detect the shared idiotype on antibodies to pseudorabies virus antigens. J Virol Methods. 1994; 48:301-313. Impact factor: 1.938. (Cited 1) 57.Zhou E-M, Dzuba-Fisher JMM, Rector ES, Sehon AH, Kisil FT. A mouse monoclonal anti-idiotypic antibody detects a common idiotope on human, mouse, and rabbit antibodies to allergen Lol pIV. Scand J Immunol. 1991; 34:307-316. Impact factor: 1.782. (Cited 2) 58.Zhou E-M, Lohman KL, Kennedy RC. Administration of noninternal image monoclonal anti-idiotypic antibodies induces idiotype-restricted responses specific for human immunodeficiency virus envelope glycoprotein epitopes. Virol. 1990; 174:9-17. Impact factor: 3.363. (Cited 5) 59.Zhou E-M, Kennedy RC. Strategies for developing vaccines against acquired immunodeficiency syndrome. Biochem Biophysica Acta Cancer Res Suppl. 1989; 986:301-313. Impact factor: 2.935. (Cited 1) 60.Zhou E-M*, Wang D-R, Kennedy RC. Anti-idiotypic antibodies as potential candidates of vaccines. Chinese J Immunol. 1988; 2:70-73. 61.Zhou E-M, Dreesman GR, Kennedy RC. Anti-idiotypic antibodies: A new generation of vaccines against infectious agents. Microbiol Sci. 1987; 4:36-40. (Cited 3) 62.Kennedy RC, Zhou E-M, Lanford RE, Chanh TC, Bona CA. Possible role of anti-idiotypic antibodies in the induction of tumor immunity. J Clin Invest. 1987; 80: 1217-1224. Impact factor: 14.051. (Cited 6) 63.Zhou E-M, Chanh TC, Dreesman GR, Kanda P, Kennedy RC. Immune response to human immunodeficiency virus: In vivo administration of anti-idiotype induces an anti-gp160 response specific for a synthetic peptide. J Immunol. 1987; 139:2950-2956. Impact factor: 7.014. (Cited 6) 64.Ren Q, Jin Z-C, Zhou E-M. Influence of uniform intensity magnetic field on the antibody forming cells in mice. J Inner Mongolia Med. 1981; 1(3):146-149. 65.王承宝,张冲(学),周恩民,赵钦,梁超(学),党露(学),马玉萍(学),高继明(外),李琼毅(学),黄柏成(学),孔宁(外);Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant;2012-08;Journal of Virology;SCI(2012)(5.402) 书章节: 66.Lowe DB, Shearer MH, Jumper CA, Zhou E-M, Kennedy. 2008. Plasmid DNA as prophylactic and therapeutic vaccines for cancer and infectious diseases. In Plasmids: Current Research and Future Trends (Georg Lipps ed.), Horizon Scientific Press. 67.Zhou E-M*, Lin M. 1997. Anti-idiotype to bluetongue virus VP7 antigen – potential candidate of diagnostic reagent and vaccine. in Idiotypes in Medicine - Infections, Autoimmunity and Cancer (Y. Shoenfeld, R.C. Kennedy and T. Ferrone ed.), Elsevier Science, The Netherlands. 68.Dreesman GR, Henkel RD, Zhou E-M, Kennedy RC. 1988. Properties of a protective anti-idiotype vaccine for viral hepatitis B. In Vaccine: New concepts and Developments (H. Kohler and P.T. LoVerde, eds.) Longman Group Ltd., UK.
科研项目: 1. 猪蓝耳病病毒感染和致病的分子机理。国家自然科学基金NSFC-广东联合基金重点项目。2010,01-2013,12. 2.猪iPS 细胞系的建立及其基因打靶。国家高技术研究发展计划(863 计划)专题课题:高效基因连续敲除技术子课题。2009,01-2011,12。 3. 高致病性蓝耳病风险分析关键技术与应用研究。国家行业科技计划项目:动物卫生风险分析关键技术与应用研究子课题。2009,01-2011,12. 4. 蓝耳病病毒新型受体突变基因的体细胞克隆。国家重大专项课题。2009,07-2010,12。 5. 新型PRRSV细胞受体鉴定及其与病毒相互作用机制的研究。国家自然基金。2009,01-2011,12. 6. 新型PRRSV淋巴细胞受体与PRRSV免疫反应的关系及抗独特性抗体对疫苗免疫的调控。兽医生物技术国家重点实验室开放课题。2009,01-2010,12。 7. 猪重大疫病防治标准化技术体系应用与示范。山东省自主创新成果转化重大专项项目。2008,01-2010,12.
荣誉及奖项: 2009年:获“第二届百名华侨华人专业人士‘杰出创业奖’” 2009年:泰安市优秀科技创新团队“动物生物技术”带头人 2008年:EPIZONE Workshop: Research in swine viral diseases “Building Bridge”分会主席 2008年:中国农业部动物疫病风险分析评估委员会委员 2007年:第十五届世界禽病大会(北京)分会主席 2006年:获黑龙江省大庆市第二十一届自然科学技术学术成果奖(禽戊型肝炎病抗原决定簇鉴定及疫苗研究) 1998年:获加拿大美洲农业总署奖励赴巴拉圭推广蓝舌病和流行性出血热酶标免疫诊断试剂以促进国际贸易 1997年:获加拿大美洲农业总署奖励赴阿根廷推广蓝舌病酶标免疫诊断试剂以促进国际贸易 1997年:第5届国际免疫学大会免疫学诊断分会主席 1994年:国际兽用疫苗大会新型疫苗分会主席 1992年:获加拿大明尼多巴省医学研究优秀研究奖 1990年:获加拿大明尼多巴大学医学研究基金最佳优秀博士研究奖 1987年:任《Viral Immunology》杂志特约审稿人 1984年:获包头医学院优秀教师奖
社会兼职: 主要学术兼职: 国家自然科学基金委员会第十三界生命科学部兽医学评审组成员,2010- 中国农业科学院学位论文评阅专家,2010- 中国农业部基因克隆与转基因技术执行专家组成员,2009- 中国农业部全国动物卫生风险评估专家委员会委员,2008- 中国畜牧兽医学会兽医公共卫生学分会常务理事,2008- 中国畜牧兽医学会高级会员,2008- 中国免疫学会高级会员,2007- 科学杂志编委及特邀审稿人: Viral Immunology Journal of Virological Methods Journal of Diary Research American Journal of Veterinary Research Transboundary and Emerging Diseases (Formerly ‘Journal of Veterinary Medicine, Series A’)
联系方式: 西北农林科技大学 陕西-杨凌-西农路22号,邮编 712100 电话:86-29-87091280; 传真:86-29-87091280 电子邮件: zhouem@nwsuaf.edu.cn
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