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李朝飞 
发布时间:2016-04-15  浏览次数:

    李朝飞,男,1973年生,陕西靖边人,教授、博士生导师,美国病毒学会会员。1996年和1999年分获沈阳农业大学农学学士和硕士学位;2003年毕业于中山大学,获微生物学专业博士学位;2003-2005年在中山大学生命科学学院/昆虫学研究所工作,讲师;2005-2011年在美国康乃尔大学Boyce Thompson植物研究所(BTI)进行博士后研究。2011年9月应聘至西北农林科技大学植物保护学院工作,同年入选教育部“新世纪优秀人才支持计划”。

主要研究方向
    昆虫病毒与分子生物学:主要研究领域包括杆状病毒与宿主细胞相互作用、病毒入侵、膜融合、以及出芽释放分子机理,昆虫病毒分离与分子进化,以及病毒杀虫剂的研制与开发等;
    昆虫细胞系及工程化:主要涉及高表达昆虫细胞系的筛选与克隆,稳定表达细胞系的构建与筛选,

以及新型昆虫表达系统的研制与开发等。

近年发表的研究论文
•Li, Z.F., Blissard, G.W. 2012. Cellular VPS4 is required for efficient entry and egress of budded virions of Autographa californica multiple nucleopolyhedrovirus.  Journal of Virology, 86(1): 459-472.
•Li, Z.F., Blissard, G.W. 2011. The AcMNPV GP64 protein: Roles of histidine residues in triggering membrane fusion and fusion pore expansion. Journal of Virology, 85(23):12492-12504.
•Li, Z.F., Blissard, G.W. 2010. Baculovirus GP64 disulfide bonds: The intermolecular disulfide bond of AcMNPV GP64 is not essential for membrane fusion and virion budding. Journal of Virology, 84(17): 8584-8595..
•Li, Z.F., Blissard, G.W. 2009. The pre-transmembrane domain of the Autographa californica multicapsid nucleopolyhedrovirus GP64 protein is critical for membrane fusion and virus infectivity. Journal of Virology, 83(21): 10993-11004.
•Li, Z.F., Blissard, G.W. 2009. The Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: analysis of transmembrane domain length and sequence requirements. Journal of Virology, 83(9): 4447-4461.
•Liu, C., Li, Z.F.*, Wu, W., Li, L.L., Yuan, M.J., Pan, L.J., Yang, K*., Pang, Y.  2008. Autographa californica multiple nucleopolyhedrovirus ac53 plays a role in nucleocapsid assembly. Virology, 382(1): 59-68. (*通讯作者).
•Li, Z.F., Blissard, G.W. 2008. Functional analysis of the transmembrane (TM) domain of Autographa californica multicapsid nucleopolyhedrovirus GP64 protein: substitution of heterologous domains. Journal of Virology, 82(7): 3329-3341.
•Wang, Y.J., Wu, W.B., Li, Z.F., Yuan, M.J., Feng, G.Z., Yu, Q., Yang, K., Pang, Y. 2007. Ac18 is not essential for the propagation of Autographa californica multiple nucleopolyhedrovirus. Virology, 367(1): 71-81.
•Wu, W.B, Lin, T.H., Pan, L.J., Yu, M., Li, Z.F., Pang, Y., Yang, K. 2006. Autographa californica multiple nucleopolyhedrovirus nucleocapsid assembly is interrupted upon deletion of the 38k gene. Journal of Virology, 80(23):11475-11485.
•Li, Z.F., Li, L.L., Yu, H., Li, S.N., Pang, Y. 2006. Characterization of two homologues of ChaB in Spodoptera litura multicapsid nucleopolyhedrovirus. Gene, 372:33-43.
•Li, Z.F., Pan, L.J., Yu, H., Li, L.L., Gong, Y.X., Yang, K., Pang, Y. 2005. Characterization of Spodoptera litura nucleopolyhedrovirus 38.7k protein, which contains a conserved BRO-N domain. Virus Research, 115(2):185-191.

联系方式
通讯地址:陕西省咸阳市杨凌区,西北农林科技大学植物保护学院6B-202室,邮编:712100
电话:029-87091650; E-mail: 09300181s@sina.cn,zhaofei_li@yahoo.com.cn

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